8536dch030659/10/0210:12 AM Page65mac46mac46:385REB Antigens cHAPTER 3 FIGURE 3-5 Computer simulation of an interaction between anti- chain is blue. (b) The complementarity of the two molecules is re- body and influenza virus antigen, a globular protein (a) The antigen vealed by separating the antigen from the antibody by 8 A.Based on (yellow) is shown interacting with the antibody molecule; the variable x-ray crystallography data collected by P. M. Colman and W.R. Tulip region of the heavy chain is red, and the variable region of the light From G. V H Nossal, 1993, Sci. Am. 269(3): 22. 1 VISUALIZING CONCEPTS (145)146-151 OOH 15-21(22) 113-119 FICURE 3-6 Protein antigens usually contain both sequential both are shown in red, blue, and white, respectively. These and nonsequential B-cell epitopes (a)Diagram of sperm whale residues are widely spaced in the amino acid sequence but are myoglobin showing locations of five sequential B-cell ep rought into proximity by folding of the protein /Part(a) adapted (blue).(b) Ribbon diagram of hen egg white lysozyme showing from M. Z. Atassi and A. L Kazim. 1978, Adv Exp. Med Biol. 98: 9, esidues that compose one nonsequential (conformational) epi- part(b) from W.G. Laver et al., 1990, Cell 61: 554. tope. Residues that contact antibody light chains, heavy chains, orAntigens CHAPTER 3 65 VISUALIZING CONCEPTS FIGURE 3-6 Protein antigens usually contain both sequential and nonsequential B-cell epitopes. (a) Diagram of sperm whale myoglobin showing locations of five sequential B-cell epitopes (blue). (b) Ribbon diagram of hen egg-white lysozyme showing residues that compose one nonsequential (conformational) epi￾tope. Residues that contact antibody light chains, heavy chains, or both are shown in red, blue, and white, respectively. These residues are widely spaced in the amino acid sequence but are brought into proximity by folding of the protein. [Part (a) adapted from M. Z. Atassi and A. L. Kazim. 1978, Adv. Exp. Med. Biol. 98:9; part (b) from W. G. Laver et al., 1990, Cell 61:554.] Heme (145) 146 −151 COOH (a) (b) NH2 15 − 21 (22) 56 − 62 113 − 119 FIGURE 3-5 Computer simulation of an interaction between anti￾body and influenza virus antigen, a globular protein. (a) The antigen (yellow) is shown interacting with the antibody molecule; the variable region of the heavy chain is red, and the variable region of the light Antigen Antibody (a) (b) chain is blue. (b) The complementarity of the two molecules is re￾vealed by separating the antigen from the antibody by 8 Å. [Based on x-ray crystallography data collected by P. M. Colman and W. R. Tulip. From G. J. V. H. Nossal, 1993, Sci. Am. 269(3):22.] 8536d_ch03_065 9/10/02 10:12 AM Page 65 mac46 mac46:385_REB: