11.2 Nucleic Acid Hybridization Nucleic acid hybridization:base pairing of single strands of DNA or RNA from two different sources to give a hybrid double helix Segment of single-stranded DNA that is used in hybridization and has a predetermined identity is called a nucleic acid probe Southern blot:a hybridization procedure where DNA is in the gel and probe is RNA or DNA Northern blot:RNA is in the gel Chen Feng,Lecture of MicrobiologyChen Feng, Lecture of Microbiology 11.2 Nucleic Acid Hybridization Nucleic acid hybridization: base pairing of single strands of DNA or RNA from two different sources to give a hybrid double helix • Segment of single-stranded DNA that is used in hybridization and has a predetermined identity is called a nucleic acid probe Southern blot: a hybridization procedure where DNA is in the gel and probe is RNA or DNA • Northern blot: RNA is in the gel