How are nmr structures solved? Solution phase technique-protein at mM concentration in a buffer. Currently limited to proteins s 30-50 kDa 2. Measure resonant frequencies of H 3C n atoms in a magnetic field 3. Assign peaks observed in the spectrum to individual amino acl 4. Measure distances between different residues 6a apart to get restraints. Need many restraints per residue. 5. Build structures consistent with the experimental distance restraints and principles of sterochemistry 6. Yields a set of structures consistent with the dataHow are NMR structures solved? 1. Solution phase technique - protein at mM concentration in a buffer. Currently limited to proteins ≤ 30-50 kDa. 2. Measure resonant frequencies of 1H, 13C, 15N atoms in a magnetic field. 3. Assign peaks observed in the spectrum to individual amino acids. 4. Measure distances between different residues < 6Å apart to get restraints. Need many restraints per residue. 5. Build structures consistent with the experimental distance restraints and principles of sterochemistry. 6. Yields a set of structures consistent with the data