C. Fluorescence Microscopy eyepiece 3 second barrier filter: cuts out unwanted fluorescent signals. passing the specific green fluorescein emission between 520 and 560 nm LIGHT SOURCE 2 bearn-splitting mirror: reflects light below 510 nm but transmits light above 510 nm 1 first barrier filter: lets through only blue light with a wavelength between 450 and 490 nm objective lens object Figure 3-7. The optical system of a modern fluorescence microscope. a filter set consists of two barrier filters(I and 3 )and a dichroic(beam- splitting) mirror(2). In this example the filter set for detection of the fluorescent molecule fluorescein is shownFigure 3-7. The optical system of a modern fluorescence microscope. A filter set consists of two barrier filters (1 and 3) and a dichroic (beam￾splitting) mirror (2). In this example the filter set for detection of the fluorescent molecule fluorescein is shown. C. Fluorescence Microscopy