Journal of Agricultural and Food Chemistry Table 1.Strains and Plasmids Used in This Study d-typ e strain,CGMCC No.083: E celi GM2163 FlaeY-galkE4 rpl.136(S)dm1:Tn NX- X-2 derivat n NX-22 of putM and NX-2-AputM n of putM gen X- utM with putM at E col and B he c twf an pa 6 Table 1. Strains and Plasmids Used in This Study strain or plasmid relevant properties source Strains B. subtilis NX-2 wild-type strain, CGMCC No.0833 CGMCC B. subtilis 168 Tthe strain with P43 promoter this lab E. coli DH5α F−,φ80dlacZΔM1, Δ(lacZYA-argF) U169, deoR, recA1, endA1, hsdR17(rk−, mk+ ), phoA, supE44, λ−thi-1, gyrA96, relA1 this lab E. coli GM2163 F−, ara-14 leuB6 thi-1 fhuA31 lacY1 tsx-78 galK2 galT22 supE44 hisG4 rpsL 136 (StrR ) xyl-5 mtl-1 dam13::Tn9 (CamR ) dcm-6 mcrB1 hsdR2 mcrA this lab NX-2-zwf NX-2 derivative, overexpression of zwf this study NX-2-pgl NX-2 derivative, overexpression of pgl this study NX-2-gnd NX-2 derivative, overexpression of gnd this study NX-2-pgi NX-2 derivative, overexpression of pgi this study NX-2-pfkA NX-2 derivative, overexpression of pfkA this study NX-2-pdhA NX-2 derivative, overexpression of pdhA this study NX-2-pdhB NX-2 derivative, overexpression of pdhB this study NX-2-pdhC NX-2 derivative, overexpression of pdhC this study NX-2-citA NX-2 derivative, overexpression of citA this study NX-2-citB NX-2 derivative, overexpression of citB this study NX-2-icd NX-2 derivative, overexpression of icd this study NX-2-sdhA NX-2 derivative, overexpression of sdhA this study NX-2-fumC NX-2 derivative, overexpression of fumC this study NX-2-gltA NX-2 derivative, overexpression of gltA this study NX-2-gltB NX-2 derivative, overexpression of gltB this study NX-2-putM NX-2 derivative, overexpression of putM this study NX-2-rocA NX-2 derivative, overexpression of rocA this study NX-2-racE NX-2 derivative, overexpression of racE this study NX-2-DegQ NX-2 derivative, overexpression of DegQ this study NX-2-DegU NX-2 derivative, overexpression of DegU this study NX-2-DegS NX-2 derivative, overexpression of DegS this study NX-2-pgsBCA NX-2 derivative, overexpression of pgsBCA this study NX-22 NX-2 derivative, combinatorial overexpression of putM and rocA this study NX-23 NX-2 derivative, combinatorial overexpression of putM, rocA and gltB this study NX-24 NX-2 derivative, combinatorial overexpression of putM, rocA, gltB and gltA this study NX-2-ΔputM NX-2 derivative, deletion of putM gene this study NX-2-ΔrocA NX-2 derivative, deletion of rocA gene this study NX-2-ΔputM-M NX-2-ΔputM derivative, complemented with putM at original locus this study NX-2-ΔrocA-A NX-2-ΔrocA derivative, complemented with rocA at original locus this study Plasmids pHY300PLK E. coli and B. subtilis shuttle vector; AmpR , TetR TaKaRa, Dalian, China pDR-pheS* pDR with P43-pheS* cassette inserted in the multicloning sites EcoRI and SpeI this laboratory pHY-zwf pHY300PLK containing P43 promoter, the gene zwf and amyL terminator this study pHY-pgl pHY300PLK containing P43 promoter, the gene pgl and amyL terminator this study pHY-gnd pHY300PLK containing P43 promoter, the gene gnd and amyL terminator this study pHY-pgi pHY300PLK containing P43 promoter, the gene pgi and amyL terminator this study pHY-pfkA pHY300PLK containing P43 promoter, the gene pfkA and amyL terminator this study pHY-pdhA pHY300PLK containing P43 promoter, the gene pdhA and amyL terminator this study pHY-pdhB pHY300PLK containing P43 promoter, the gene pdhB and amyL terminator this study pHY-pdhC pHY300PLK containing P43 promoter, the gene pdhC and amyL terminator this study pHY-citA pHY300PLK containing P43 promoter, the gene citA and amyL terminator this study pHY-citB pHY300PLK containing P43 promoter, the gene citB and amyL terminator this study pHY-icd pHY300PLK containing P43 promoter, the gene icd and amyL terminator this study pHY-sdhA pHY300PLK containing P43 promoter, the gene sdhA and amyL terminator this study pHY-fumC pHY300PLK containing P43 promoter, the gene fumC and amyL terminator this study pHY-gltA pHY300PLK containing P43 promoter, the gene gltA and amyL terminator this study pHY-gltB pHY300PLK containing P43 promoter, the gene gltB and amyL terminator this study pHY-putM pHY300PLK containing P43 promoter, the gene putM and amyL terminator this study pHY-rocA pHY300PLK containing P43 promoter, the gene rocA and amyL terminator this study pHY-racE pHY300PLK containing P43 promoter, the gene racE and amyL terminator this study pHY-DegQ pHY300PLK containing P43 promoter, the gene DegQ and amyL terminator this study pHY-DegU pHY300PLK containing P43 promoter, the gene DegU and amyL terminator this study pHY-DegS pHY300PLK containing P43 promoter, the gene DegS and amyL terminator this study Journal of Agricultural and Food Chemistry Article DOI: 10.1021/acs.jafc.9b01755 J. Agric. Food Chem. 2019, 67, 6263−6274 6264