Acid hydrolysis ofproteins 6N HCI 110C 24 or 48 or 72hr Trp Ser thr Asn gin H HH H H H N- N--c-coo H3N-C-CO0+'H:3N-C-Co0 R O R R HPLC or ion-exchange 6M HCI Free amino chromatography Amino acid acids com position PolypeptideAcid hydrolysis of proteins • 6N HCl 110℃ 24 or 48 or 72hr • Trp • Ser Thr • Asn Gln