Distilled(蒸馏水)1000mlpH7.0 适用范围:大肠埃希氏菌(大肠杆菌)、恶臭假单胞菌 33. LB Medium(LB培养基) Yeast extact(酵母膏)5 g Peptone(蛋白胨)10g NaCl10 g Agar(琼脂)1-2% Distilled water(蒸馏水)1000mlpH7.0 适用范围:大肠埃希氏菌(大肠杆菌) 34. Penssay Broth( Penssay肉汤) Beef extract(牛肉膏)1.5 g Yeast extract(酵母膏)1.5g Tryptone(胰蛋白胨)5gNaC13.5g Glucose(葡萄糖)1gK2HPO4.3H204.8g Distilled water(蒸馏水)1000m1pH7.2 35. Blond Agar(血琼脂培养基) Peptone(蛋白胨)l0 g Glucose(葡萄糖)2g Nacl 5g NaH2P04 2 5g Sodium citrate(柠檬酸钠)5 g Brain infusion(脑浸汁)800r Pig heart infusion(猪心浸)200 mI Sleep blood(羊血)10% pH7.2 [Note]: Preparation of pig brain and pig heart infusion: (1) Smash up 100g fresh pig heart (without fat and blood vessel).Add distilled water up to 1000ml )Cut 200g fresh pig brain(without blood vessel). Add distilled water o1000ml; (3) Keep the two infusions at 50C for 1 hr, then boil for 15 minutes (4) Cool and filtrate through gauze.[注]猪心和猪脑浸汁法:(1)新 鲜猪心除去心头和脂肪,搅碎100克加蒸馏水至1000毫升;(2)新鲜猪脑除去 血管,取200克加蒸馏水至1000毫升;(3)50℃保温1小时,然后煮开15分 钟;(4)冷却后用纱布过滤备用。 36. Cellulose- decomposing Bacteria Synthetic Medium(纤维细菌合成培养 基) Nacl 6g MgS04. 7H20 0. lg KH2P040.5gCaC120.1 K2HPO42.0 g Yeast extract(酵母膏)1.0g (NH4)2S042.0 g Distilled water(蒸馏水)1000ml Filter paper strip: one strip(滤纸条:一片)pH7.0-7.4 Note]: So; id medium for cellulose-decomposing bacteria: Carboxymethyl Cellulose2%Agar%.(斜面培养基去滤纸条用2%琼脂及2%羧甲基纤维素钠 CMCNaDistilled (蒸馏水) 1000ml pH 7.0 适用范围：大肠埃希氏菌（大肠杆菌）、恶臭假单胞菌 33. LB Medium (LB 培养基) Yeast extact (酵母膏) 5g Peptone (蛋白胨) 10g NaCl 10g Agar (琼脂) 1-2% Distilled water (蒸馏水) 1000ml pH 7.0 适用范围：大肠埃希氏菌（大肠杆菌） 34. Penssay Broth (Penssay 肉汤) Beef extract (牛肉膏) 1.5g Yeast extract (酵母膏) 1.5g Tryptone (胰蛋白胨) 5g NaCl 3.5g Glucose (葡萄糖) 1g K2HPO4.3H2O 4.8g Distilled water (蒸馏水) 1000ml pH 7.2 35. Blond Agar (血琼脂培养基) Peptone (蛋白胨) 10g Glucose (葡萄糖) 2g NaCl 5g NaH2PO4 2.5g Sodium citrate (柠檬酸钠) 5g Brain infusion (脑浸汁) 800ml Pig heart infusion (猪心浸) 200ml Sleep Blood (羊血) 10% pH 7.2 [Note]：Preparation of pig brain and pig heart infusion： （1） Smash up 100g fresh pig heart （without fat and blood vessel）.Add distilled water up to 1000ml; （2） Cut 200g fresh pig brain (without blood vessel). Add distilled water up to 1000ml; （3） Keep the two infusions at 50℃ for 1 hr , then boil for 15 minutes; （4） Cool and filtrate through gauze . [注]猪心和猪脑浸汁法：（1）新 鲜猪心除去心头和脂肪，搅碎 100 克加蒸馏水至 1000 毫升；（2）新鲜猪脑除去 血管，取 200 克加蒸馏水至 1000 毫升；（3）50℃保温 1 小时，然后煮开 15 分 钟；（4）冷却后用纱布过滤备用。 36. Cellulose﹣decomposing Bacteria Synthetic Medium (纤维细菌合成培养 基) NaCl 6g MgSO4.7H2O 0.1g KH2PO4 0.5g CaCl2 0.1g K2HPO4 2.0g Yeast extract (酵母膏) 1.0g (NH4)2SO4 2.0g Distilled water (蒸馏水) 1000ml Filter paper strip: one strip (滤纸条：一片) pH 7.0-7.4 [Note]: So;id medium for cellulose-decomposing bacteria: Carboxymethyl Cellulose 2% Agar 2%. （斜面培养基去滤纸条用 2%琼脂及 2%羧甲基纤维素钠 CMCNa ）