Question 4 Working in mice, you discover a gene, LCl, that when mutant decreases the incidence of cancer in mice. This gene is normally expressed in the lymphatic tissues of mice. You know the sequence of this gene, and you really want to see if there is a similar gene is in humans. So, you set out to find the human homolog of LC1 by by screening a human librar a)What kind of human library would you use? Explain Because the lci gene may only be expressed in the lymphatic tissues of mice, you may choose to screen a genomic library. If you were to screen a cDNA library, you would need to make sure that lymphatic specific mRNA's were represented b)How would you screen the human library for the homolog of LC1? Because you know the sequence of the lci gene from mice, you could make a probe complementary to hybridization could be performed at a temperature that would allow probe binding to similar but pa the mouse sequence. A human homolog, by definition, would have significant sequence similarity necessarily identical sequence 7012Fall20037.012 Fall 2003 5 Question 4 Working in mice, you discover a gene, LC1, that when mutant decreases the incidence of cancer in mice. This gene is normally expressed in the lymphatic tissues of mice. You know the sequence of this gene, and you really want to see if there is a similar gene is in humans. So, you set out to find the human homolog of LC1 by screening a human library. a) What kind of human library would you use? Explain. Because the LC1 gene may only be expressed in the lymphatic tissues of mice, you may choose to screen a genomic library. If you were to screen a cDNA library, you would need to make sure that lymphatic specific mRNA’s were represented. b) How would you screen the human library for the homolog of LC1? Because you know the sequence of the LC1 gene from mice, you could make a probe complementary to the mouse sequence. A human homolog, by definition, would have significant sequence similarity. The hybridization could be performed at a temperature that would allow probe binding to similar but not necessarily identical sequence