Question 6 A long time ago, in a galaxy far far away, you are a scientist. you have just joined a lab that recently published a paper in Galactic Scientific reporting a new microorganism named Metachlorianus forcus, called metachlorians. These microorganisms colonize the brains of most humanoids. However, in those who are part of the religious sect known as the Jedi, they are found in very high concentrations. In Jedi, these microorganisms secrete a protein known as Saberin Saberin is encoded by the gene saberin. Saberin, when released in the brain, makes the Jedi emanate a sword known to many as a light saber blue is the most common light saber color amongst the jedi you would like to understand this phenomenon. First, you study the metachlorians. Like present day yeast cells, the metachlorians can live in both a diploid and haploid state. Knowing this, you can now study the genetics of the light saber phenomenon The assay you mutagenize haploid metachlorians and isolate mutants altered in the light saber phenomenon. The way you are going to look for mutants is by injecting mutagenized strains into the brain of a willing Jedi, Jacensolo, who has been depleted of his own metachlorians Because the strains you will inject are growth impaired, they stay around only long enough for jacensolo to make one light saber. This allows you to follow the protocol shown Inject mutant strain of metachlorians into the brain of jacensolo Allow Jacensolo to make a light saber Check light saber color for non-blue colors Let the injected metachlorians die off Inject a new mutant strain In this procedure, you isolate 10 mutants: m1, m2, m3, m4, m5, m6, m7, m8, m9 and m10. All of these mutants cause Jacensolo to create light sabers that are not blue The first thing you do is to find out how many complementation groups exist. So, you make diploid combinations between each of the mutants. you then inject these diploids into jacensolo and see if he produces a blue(WT)light saber. The results are shown below (+)=Blue light saber (-) non-blue light saber ml m2 m3 m4 m5 m6 m7 m8 m9 m10 wt m10 77 Question 6 A long time ago, in a galaxy far, far away, you are a scientist. You have just joined a lab that recently published a paper in Galactic Scientific reporting a new microorganism named Metachlorianus forcus, called metachlorians. These microorganisms colonize the brains of most humanoids. However, in those who are part of the religious sect known as the Jedi, they are found in very high concentrations. In Jedi, these microorganisms secrete a protein known as Saberin. Saberin is encoded by the gene saberin. Saberin, when released in the brain, makes the Jedi emanate a sword known to many as a light saber. Blue is the most common light saber color amongst the Jedi. You would like to understand this phenomenon. First, you study the metachlorians. Like present day yeast cells, the metachlorians can live in both a diploid and haploid state. Knowing this, you can now study the genetics of the light saber phenomenon. The assay: You mutagenize haploid metachlorians and isolate mutants altered in the light saber phenomenon. The way you are going to look for mutants is by injecting mutagenized strains into the brain of a willing Jedi, Jacensolo, who has been depleted of his own metachlorians. Because the strains you will inject are growth impaired, they stay around only long enough for Jacensolo to make one light saber. This allows you to follow the protocol shown: • Inject mutant strain of metachlorians into the brain of Jacensolo • Allow Jacensolo to make a light saber • Check light saber color for non-blue colors • Let the injected metachlorians die off • Inject a new mutant strain In this procedure, you isolate 10 mutants: m1, m2, m3, m4, m5, m6, m7, m8, m9 and m10. All of these mutants cause Jacensolo to create light sabers that are not blue. The first thing you do is to find out how many complementation groups exist. So, you make diploid combinations between each of the mutants. You then inject these diploids into Jacensolo and see if he produces a blue (WT) light saber. The results are shown below: (+) = Blue light saber (-) = non-blue light saber m1 m2 m3 m4 m5 m6 m7 m8 m9 m10 wt m1 --- --- +++ +++ +++ +++ --- +++ +++ --- +++ m2 --- +++ +++ +++ +++ --- +++ +++ --- +++ m3 --- +++ +++ --- +++ --- +++ --- +++ m4 --- --- +++ +++ +++ --- --- +++ m5 --- +++ +++ +++ --- --- +++ m6 --- +++ --- +++ --- +++ m7 --- +++ +++ --- +++ m8 --- +++ --- +++ m9 --- --- +++ m10 --- ---