Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S Gerstein opyright◎2001 ISBNS:0-471-37972-7( Paper);0-471 (Electronic) 2 Electrophoresis Martha L booz Chemical Safety What ls the Safest Approach to Working with 334 What Are the Symptoms of Acrylamide Poisoning?.... 335 What Is the Medical Response to Accidental Acrylamide ..335 What ls the Shelf Life of Acrylamide and Acrylamide e?.335 How Can You Dispose of Excess, Unusable Acrylam Solutions? Electrical Safety ..336 What Are the Requirements for Safe Equipment in Good 336 What Are the Requirements for a Safe Work area? Working Order 337 Polyacrylamide(PAGE) Gels--Before Selecting a Gel Getting the best results for Your 337 What Is the Mechanism of Acrylamide Polymerization?.. 338 What Other Crosslinkers are available. and when Should They Be Used? 338 How Do you control pore size How Do You calculate %T and %C? 34 I am grateful to Bruce Goodrich for the figure on degassing acrylamide, to Fiona Leung for the data regarding the molecular weight vs. relative mobility curve, and to Lee olech and Dave Garfin for fruitful discussions about many of the questions in this chapter.331 12 Electrophoresis Martha L. Booz Chemical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 334 What Is the Safest Approach to Working with Acrylamide? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 334 What Are the Symptoms of Acrylamide Poisoning? . . . . . . 335 What Is the Medical Response to Accidental Acrylamide Exposure? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 335 How Can You Dispose of Excess, Unusable Acrylamide? . . 335 What Is the Shelf Life of Acrylamide and Acrylamide Solutions? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 336 Electrical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 336 What Are the Requirements for a Safe Work Area? . . . . . . 336 What Are the Requirements for Safe Equipment in Good Working Order? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 337 Polyacrylamide (PAGE) Gels—Before Selecting a Gel: Getting the Best Results for Your Purpose . . . . . . . . . . . . . . . . 337 What Is the Mechanism of Acrylamide Polymerization? . . . 338 What Other Crosslinkers Are Available, and When Should They Be Used? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 338 How Do You Control Pore Size? . . . . . . . . . . . . . . . . . . . . . . 339 How Do You Calculate %T and %C? . . . . . . . . . . . . . . . . . . . 341 I am grateful to Bruce Goodrich for the figure on degassing acrylamide, to Fiona Leung for the data regarding the molecular weight vs. relative mobility curve, and to Lee Olech and Dave Garfin for fruitful discussions about many of the questions in this chapter. Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 2001 by Wiley-Liss, Inc. ISBNs: 0-471-37972-7 (Paper); 0-471-22390-5 (Electronic)