Some typical signal peptides Function of Signal peptide Sign Import inteR e.g.TH3N-Met-Met-Ser-Phe-Val-Ser-Leu-Ler-Leu-Val-Gly-Ile-Ler Phe-Trp-Ala-Thr-Glu-Ala-Glu-GIn-Leu-Thr-Lys-Cys-Glu-Val-Phe (5-10 residues Retain in Lys-Asp-Glu-Leu-COO(KDEL) lumen of er Import into Mit H3N-Met-Leu-Ser-Leu-Arg-Gln-Ser-lle-Arg-Phe-Phe-Lys-Pr o-Ala-Thr-Arg-Thr-Leu-Cys-Ser-Ser-Arg-Tyr-Leu-Leu- Import into and e.g. for SV40T antigen, -Pro-Pro-Lys-Lys-Arg-Lys-Val-(NLS export out of nucleus nuclear-localiz ignal, rich in Lys and Arg basic aa)versus NES (leu- rich nuclear -export signal Import into e.g.-Ser-Lys-Leu- peroxisome Attach to membranes H,N- Gly-Ser-Ser-Lys-Ser-Lys-Pro-Lys- of myristic acid to the N-endSome typical signal peptides 信号功能 Attach to membranes via the covalent linkage of myristic acid to the N-end + H3N- Gly-Ser-Ser-Lys-Ser-Lys-Pro-Lys- Function of signal peptide Signal peptide Import intoER e.g. +H3N-Met-Met-Ser-Phe-Val-Ser-Leu-Ler-Leu-Val-Gly-Ile-Leu￾Phe-Trp-Ala-Thr-Glu-Ala-Glu-Gln-Leu-Thr-Lys-Cys-Glu-Val-Phe -Gln (5-10 hydrophobic residues among 15-06 residues)Hrere3N-Met-Met-Ser-Phe-Val-Ser-Leu-Ler-Leu-Val-Gly￾Ile-Leu-Phe-Trp-Ala-Thr-Glu-Ala-Glu-Gln-Leu-Thr￾Lys-Cys-Glu-Val-Phe-Gln￾Retain in lumen of ER -Lys-Asp-Glu-Leu-COO- (KDEL) Import into Mit. e.g. +H3N-Met-Leu-Ser-Leu-Arg-Gln-Ser-Ile-Arg-Phe-Phe-Lys-Pr o-Ala-Thr-Arg-Thr-Leu-Cys-Ser-Ser-Arg-Tyr-Leu-Leu- ( N-end-[positive charged residue-hydrophobic residues]n-) Import into and export out of nucleus e.g. for SV40T antigen,-Pro-Pro-Lys-Lys-Arg-Lys-Val- (NLS, nuclear-localization signal, rich in Lys and Arg basic aa) versus NES (leu-.rich nuclear-export signal) Import into peroxisome e.g.-Ser-Lys-Leu-