Name ection: Question 2, continued Your friend, Dr. Tantivy frequently works with mice, but unfortunately is both clumsy and forgetful and the mice often escape and hide in the dark corners of the room. To make them easier to find, he has engineered a retrovirus that carries a gene that when expressed glows softly green. The virus efficiently infects keratinocytes in the epidermis, and can, on occasion, stably integrate itself into the host cell genome By infecting adult mice with the virus, he can make them glow green. d)The new glo e"are indeed much easier to catch, but they are also beginning to look tired and unhappy and Dr. Tantivy realizes that their glow is keeping them up at night Luckily, he designed the retrovirus to only infect terminally differentiated keratinocytes. B treating the mice with a drug that inhibits retroviral replication, he believes he can rid the mice of virus even though it may have stably integrated itself into the host cell genome. What's his reasoning behind this strategy e)After the treatment, the mice become refreshingly dim for a few days, but then, much to everyone's dismay, one of the mice relapses and lights up again. When Dr. Tantivy compares this recurrent virus strain to the original one he finds that it is additionally infecti population of epidermal cells. How might this explain why the treatment failed ing a new 7012Fall2003Name:___________________________________ Section:_____ 7.012 Fall 2003 4 Question 2, continued Your friend, Dr. Tantivy frequently works with mice, but unfortunately is both clumsy and forgetful and the mice often escape and hide in the dark corners of the room. To make them easier to find, he has engineered a retrovirus that carries a gene that when expressed, glows softly green. The virus efficiently infects keratinocytes in the epidermis, and can, on occasion, stably integrate itself into the host cell genome. By infecting adult mice with the virus, he can make them glow green. d) The new "glow-mice" are indeed much easier to catch, but they are also beginning to look tired and unhappy and Dr. Tantivy realizes that their glow is keeping them up at night. Luckily, he designed the retrovirus to only infect terminally differentiated keratinocytes. By treating the mice with a drug that inhibits retroviral replication, he believes he can rid the mice of virus even though it may have stably integrated itself into the host cell genome. What's his reasoning behind this strategy? e) After the treatment, the mice become refreshingly dim for a few days, but then, much to everyone's dismay, one of the mice relapses and lights up again. When Dr. Tantivy compares this recurrent virus strain to the original one, he finds that it is additionally infecting a new population of epidermal cells. How might this explain why the treatment failed?