L.S. Azad et al. Fish& Shellfish Immunology 23(2007)154-163 Table ANOVA of ELISA titres in milkfish fed Vitamins( C and E)-supplemented diets Source of variatio df P- value 0.009471 0.002368 83E-41 462613 0.006016 0.001504 9591786 41E33 2.462613 0.003905 0.000244 15.56677 1.88E-20 1745647 0.001568 57E05 Total 0.02096 higher levels of Vitamin E(T4) produced growth retardation. Higher levels of vitamins are required by fish in tropical aquaculture due to increased physiological stress [21, 22]. Enhanced growth and survival responses of hybrid striped bass juveniles fed vitamins C and e was noticed by Sealy and Galtin [23]. It is evident from the present study that the supra dietary levels of both vitamins C and e resulted in a better survival response of milkfish juveniles compared to he fish in control group e, Role of dietary vitamins in the context of disease resistance of farmed fish has been very well established. Ascorbic deficiency in rainbow trout[24], channel catfish [25] and Atlantic salmon [26] was found to increase disease ole comparisons (Tukey's HSD) of mean ELISA titres in different treatments at different time intervals after priming or booster vaccination st-priming/booster keys HSD'q Treatments Difference betwee TI T2 0 dpp 0.00725 0.0004 0.002 0.0008 0.0016 0.0004 0.0012 7 dpp 0.00738 2BTT 0 -0.0052 0.0022 0.0104* 0.0074* 0.0156* 0.018* 14 dpp 0.00511 0.013 0.01 0.0234 345 21 dpp 0.00579 0.006 345 42 dpp 0.01065 0.006 -0.0066 -0.0006 7 dpb 0.01045 -0.0048 0.004 0.0008 0.0152* 0012 0.003462 0.0112* 0.006higher levels of Vitamin E (T4) produced growth retardation. Higher levels of vitamins are required by fish in tropical aquaculture due to increased physiological stress [21,22]. Enhanced growth and survival responses of hybrid striped bass juveniles fed vitamins C and E was noticed by Sealy and Galtin [23]. It is evident from the present study that the supra dietary levels of both vitamins C and E resulted in a better survival response of milkfish juveniles compared to the fish in control group. Role of dietary vitamins in the context of disease resistance of farmed fish has been very well established. Ascorbic acid deficiency in rainbow trout [24], channel catfish [25] and Atlantic salmon [26] was found to increase disease Table 3a ANOVA of ELISA titres in milkfish fed Vitamins (C and E)-supplemented diets Source of variation SS df MS F P-value F crit Duration 0.009471 4 0.002368 151.002 1.83E-41 2.462613 Treatments 0.006016 4 0.001504 95.91786 2.41E-33 2.462613 Interaction 0.003905 16 0.000244 15.56677 1.88E-20 1.745647 Within 0.001568 100 1.57E-05 Total 0.02096 124 Table 3b Multiple comparisons (Tukey’s HSD) of mean ELISA titres in different treatments at different time intervals after priming or booster vaccination Days post-priming/booster Tukey’s HSD ‘q’ Treatments Difference between mean antibody titres T1 T2 T3 T4 T5 0 dpp 0.00725 T1 0 0.0004 0.002 0.0008 0.0012 T2 0 0.0016 0.0004 0.0008 T3 0 0.0012 0.0008 T4 0.0004 T5 0 7 dpp 0.00738 T1 0 0.0052 0.0022 0.0104* 0.0128* T2 0 0.0074* 0.0156* 0.018* T3 0 0.0082* 0.0106* T4 0 0.0024 T5 0 14 dpp 0.00511 T1 0 0.013* 0.003 0.0076* 0.0104* T2 0 0.01* 0.0206* 0.0234* T3 0 0.0106* 0.0134* T4 0.0028 T5 0 21 dpp 0.00579 T1 0 0.006* 0.0052 0.003 0.0072* T2 0 0.0008 0.009* 0.0132* T3 0 0.0082* 0.0124* T4 0 0.0042 T5 0 42 dpp 0.01065 T1 0 0.006 0.0066 0.0058 0.007 T2 0 0.0006 0.0118* 0.013* T3 0 0.0124* 0.0136* T4 0 0.0012 T5 0 7 dpb 0.01045 T1 0 0.0048 0.004 0.0104 0.00672 T2 0 0.0008 0.0152* 0.01152* T3 0 0.0144 0.01072 T4 0 0.00368 T5 0 21 dpb 0.003462 T1 0 0.0112* 0.006* 0.0203* 0.0238* T2 0 0.0052* 0.0315* 0.035* T3 0 0.0263* 0.0298* T4 0 0.0035* T5 0 *Significant at P ¼ 0.05. I.S. Azad et al. / Fish & Shellfish Immunology 23 (2007) 154e163 159