Ex Intron E XOn 22. 4 The JACUAAC- Py-AG. Left Branch Pytract Right consensus consensus spliceosome AS F/F2 contains snrNas U binds 5 spice site ATP hydrolyzed u2AF binds pyrimidine tra d Figure 22. 10 The ●AsF2- UACU AA Py-AG→ com ple 2 binds branch site splicing reaction OU2AF proceeds through 日 complex L5]4/6 tri mer binds 5 binds exonat 5 ste discrete stages in L6 binds U2 which spliceosome U6 B2 complex sed formation involves at 5'splce site ATP hydrolyzed the interaction of UACUAAC P U4 is released components that U6/2 catty zes transeserifi catio ATP hydrolyzed U5 binds exon at 3 splice site site cleaved lariat for me recognIze the consensus UACUA U2/5/6 rem ain boundto lariat 3 site cleaved exonsligated sequences U UACUAAC--Py-AG Spliced RNA, is released 请莘大 Lariat debranchedFigure 22.10 The splicing reaction proceeds through discrete stages in which spliceosome formation involves the interaction of components that recognize the consensus sequences. 22.4 The spliceosome contains snRNAs