Procedure Collect. store and transfer the specimens Separation culture(streaking on plate) Gram staining Pick up predominant— Inoculation in isolates cooked meal medium Inoculate on biochemistry slip, Antibiotic susceptibility tests Judgment on the resultsProcedure Collect, store and transfer the specimens Separation culture (streaking on plate) Pick up predominant isolates Gram staining Inoculation in cooked meal medium Inoculate on biochemistry slip, Antibiotic susceptibility tests Judgment on the results