工程科学学报，第39卷.第10期：1498-1506,2017年10月 Chinese Journal of Engineering,Vol.39,No.10:1498-1506,October 2017 D0L:10.13374/j.issn2095-9389.2017.10.006;htp:/journals..usth.edu.cn 次生硫化铜矿微生物浸出实验 尹升华12,3》，王雷鸣123)四，潘晨阳12)，陈勋23)，谢芳芳2)，艾纯明4) 1)北京科技大学土木与资源工程学院，北京100083 2)北京科技大学金属矿山高效开采与安全教有部重点实验室，北京100083 3)北京金诚信矿山技术研究院有限公司，北京101500 4)辽宁工程技术大学安全科学与工程学院，葫芦岛125105 ☒通信作者，E-mail:1227540389@q4.cm 摘要微生物浸矿是提取低品位，难选次生硫化铜矿中有价元素的最有效方法之一，本研究利用嗜酸氧化亚铁硫杆菌 (Acidthiobacillus ferrooxidans)浸取福建某难选次生硫化铜矿，依次开展浸矿菌富集培养实验、驯化转代实验和不同粒径配比下 柱浸试验，获得了不同阶段的细菌浓度、H值、铜浸出率等演变规律：并结合电子计算机断层扫描技术实现了柱内矿旷堆塌落、 截面孔隙演化和浸矿机理研究.研究表明：细菌浓度和H值均呈现缓慢增加后趋降低的趋势，浸柱中细菌增殖较慢，浸矿 480h后，细菌浓度仅为每毫升5×10个，浸矿过程中，细颗粒趋于向柱底迁移，矿堆出现塌落：柱顶孔隙率变大，增幅为 6.65%,柱底孔隙率变小，降幅为8.29%：塌落程度与细粒含量成正比，最小塌落为1.7mm,最大塌落为6.15mm.入堆矿石粒 径极大影响着柱浸体系的浸出效果.实验中柱浸B组（粒径r<1mm占28.41%）浸矿效果最佳，浸矿480h后铜浸出率达 47.23%. 关键词次生硫化铜矿：嗜酸氧化亚铁硫杆菌：摇瓶：柱浸：铜浸出率 分类号TD853.3 Secondary copper sulfide bioleaching experiments YIN Sheng-hua2),WANG Lei-ming),PAN Chen-yang2),CHEN Xun'2),XIE Fang-fang?),Al Chun-ming) 1)School of Civil and Resource Engineering,University of Science and Technology Beijing,Beijing 100083,China 2)Key Laboratory of Ministry of Education for High-Efficient Mining and Safety of Metal,University of Science and Technology Beijing,Beijing 100083. China 3)Beijing JCHX Mine Technology Research Institute Co.Ltd,Beijing 101500,China 4)College of Safety Science and Engineering,Liaoning Technical University,Huludao 125105,China Corresponding author,E-mail:1227540389@qq.com ABSTRACT Bioleaching is one of the most effective methods of extracting valuable elements from low-grade,refractory secondary copper sulfide.In this research,Acidithiobacillus ferrooxidans was used to leach refractory secondary copper sulfide from Fujian Prov- ince.The bioleaching microorganisms were added to the culture,domesticated,and column leaching experiments.These were based on different particle size ratios and carried out sequentially.Based on this experiments,the evolution disciplinarians of bacterial con- centration,pH values and copper extraction rates were obtained at different stages.Using computed tomography (CT)technology,ore heap slumping inside the columns,cross section porosity evolution and leaching mechanisms were studied.The results indicate that bacterial concentration and pH values present trends that increase first and then stabilize.The proliferation of bacteria in the leaching columns is slower and the bacterial concentration is only 5x 10'per mL after 480h.During the leaching process,fine particles tend to move to the bottom of columns and the ore heap slump phenomenon appears.On the top surface of the column the porosity tends to be 收稿日期：2016-03-26 基金项目：国家自然科学基金资助项目(51374035,51574013,51604138)：新世纪优秀人才支持计划资助项目(NCET-13-0669);全国优秀博 士学位论文作者专项资金资助项目(201351)工程科学学报,第 39 卷,第 10 期:1498鄄鄄1506,2017 年 10 月 Chinese Journal of Engineering, Vol. 39, No. 10: 1498鄄鄄1506, October 2017 DOI: 10. 13374 / j. issn2095鄄鄄9389. 2017. 10. 006; http: / / journals. ustb. edu. cn 次生硫化铜矿微生物浸出实验 尹升华1,2,3) , 王雷鸣1,2,3) 苣 , 潘晨阳1,2) , 陈 勋1,2,3) , 谢芳芳2) , 艾纯明4) 1) 北京科技大学土木与资源工程学院, 北京 100083 2) 北京科技大学金属矿山高效开采与安全教育部重点实验室, 北京 100083 3)北京金诚信矿山技术研究院有限公司, 北京 101500 4)辽宁工程技术大学安全科学与工程学院, 葫芦岛 125105 苣通信作者, E鄄mail: 1227540389@ qq. com 收稿日期: 2016鄄鄄03鄄鄄26 基金项目: 国家自然科学基金资助项目(51374035, 51574013, 51604138);新世纪优秀人才支持计划资助项目(NCET鄄鄄13鄄鄄0669);全国优秀博 士学位论文作者专项资金资助项目(201351) 摘 要 微生物浸矿是提取低品位,难选次生硫化铜矿中有价元素的最有效方法之一. 本研究利用嗜酸氧化亚铁硫杆菌 (Acidthiobacillus ferrooxidans)浸取福建某难选次生硫化铜矿,依次开展浸矿菌富集培养实验、驯化转代实验和不同粒径配比下 柱浸试验,获得了不同阶段的细菌浓度、pH 值、铜浸出率等演变规律;并结合电子计算机断层扫描技术实现了柱内矿堆塌落、 截面孔隙演化和浸矿机理研究. 研究表明:细菌浓度和 pH 值均呈现缓慢增加后趋降低的趋势,浸柱中细菌增殖较慢,浸矿 480 h 后,细菌浓度仅为每毫升 5 伊 10 7 个. 浸矿过程中,细颗粒趋于向柱底迁移,矿堆出现塌落;柱顶孔隙率变大,增幅为 6郾 65% ,柱底孔隙率变小,降幅为8郾 29% ;塌落程度与细粒含量成正比,最小塌落为1郾 7 mm,最大塌落为6郾 15 mm. 入堆矿石粒 径极大影响着柱浸体系的浸出效果. 实验中柱浸 B 组(粒径 r < 1 mm 占 28郾 41% )浸矿效果最佳,浸矿 480 h 后铜浸出率达 47郾 23% . 关键词 次生硫化铜矿; 嗜酸氧化亚铁硫杆菌; 摇瓶; 柱浸; 铜浸出率 分类号 TD853郾 3 Secondary copper sulfide bioleaching experiments YIN Sheng鄄hua 1,2,3) , WANG Lei鄄ming 1,2,3) 苣 , PAN Chen鄄yang 1,2) , CHEN Xun 1,2,3) , XIE Fang鄄fang 2) , AI Chun鄄ming 4) 1) School of Civil and Resource Engineering, University of Science and Technology Beijing, Beijing 100083, China 2) Key Laboratory of Ministry of Education for High鄄Efficient Mining and Safety of Metal, University of Science and Technology Beijing, Beijing 100083, China 3) Beijing JCHX Mine Technology Research Institute Co. Ltd, Beijing 101500, China 4) College of Safety Science and Engineering, Liaoning Technical University, Huludao 125105, China 苣 Corresponding author, E鄄mail: 1227540389@ qq. com ABSTRACT Bioleaching is one of the most effective methods of extracting valuable elements from low鄄grade, refractory secondary copper sulfide. In this research, Acidithiobacillus ferrooxidans was used to leach refractory secondary copper sulfide from Fujian Prov鄄 ince. The bioleaching microorganisms were added to the culture, domesticated, and column leaching experiments. These were based on different particle size ratios and carried out sequentially. Based on this experiments, the evolution disciplinarians of bacterial con鄄 centration, pH values and copper extraction rates were obtained at different stages. Using computed tomography (CT) technology, ore heap slumping inside the columns, cross section porosity evolution and leaching mechanisms were studied. The results indicate that bacterial concentration and pH values present trends that increase first and then stabilize. The proliferation of bacteria in the leaching columns is slower and the bacterial concentration is only 5 伊 10 7 per mL after 480 h. During the leaching process, fine particles tend to move to the bottom of columns and the ore heap slump phenomenon appears. On the top surface of the column the porosity tends to be