http://micro.magnetfsuedu/primer/java/microscopy/immersion/index.html Resolution of a Microscope (lateral) The smallest distance between two specimen points that can still be distinguished as two separate entities dmin =0.61\/NA NA=nsin(a) 入-ⅲ urination wavelength(ight) NA -numerical aperture a-one half of the objective angular aperture n-imaging medium refractive index dmin n 0. 3um for a midspectrum 2 of 0. 55um https://www.youtube.com/watch?v=nzasdncmymoatn5:35-6:00The smallest distance between two specimen points that can still be distinguished as two separate entities dmin = 0.61l/NA NA=nsin() l – illumination wavelength (light) NA – numerical aperture -one half of the objective angular aperture n-imaging medium refractive index dmin ~ 0.3m for a midspectrum l of 0.55m Resolution of a Microscope (lateral) http://micro.magnet.fsu.edu/primer/java/microscopy/immersion/index.html https://www.youtube.com/watch?v=n2asdncMYMo at~5:35-6:00