DETERMINATION OF CHLOROFORM EXTRACTABLE FATTY SUBSTANCES IN POTATO GRANULES Whatman Extraction Thimble 30mm by 100mm (41 by 123mm) Analytical Balance Cotton Wool Quickfit Round Bottom Flask 250ml (500ml) Soxhlet extractor Soxhlet Condenser Isomantle Drying Oven100℃ Desiccator Desiccant Reagents Petroleum Spirit(40"-60C) Method Leigh accurately, approximately 20g (50g) sample into an extraction thimble 30 by 100mm(41 by 123mm) 2. Plug with wad of cotton wool (fat-free) 3. Weigh accurately a clean dry 250ml (550ml) Round Bottom Flask. 4. Pour 150ml (300ml)Petroleum Spirit into the flask 5. Place the thimble containing the sample into a Soxhlet extractor 6. Connect the Soxhlet extractor to the flask and a condenser. 7. Place in an isomantle 8. Extract for at least 4hr. 9. Distil off Petroleum Spirit 10. Place the flask in the drying oven for 1hr. 11. Remove and place in the desiccator to cool 12. Reweigh the flask Calculation Extractable Fat Increase in weight of flask x 100 Weight of SampleDETERMINATION OF CHLOROFORM EXTRACTABLE FATTY SUBSTANCES IN POTATO GRANULES Apparatus Whatman Extraction Thimble 30mm by 100mm (41 by 123mm) Analytical Balance Cotton Wool Quickfit Round Bottom Flask 250ml. (500ml). Soxhlet Extractor Soxhlet Condenser Isomantle Drying Oven 100°C Desiccator & Desiccant Reagents Petroleum Spirit (40" - 60°C). Method 1. Weigh accurately, approximately 20g (50g) sample into an extraction thimble 30 by lOOmm (41 by 123mm). 2. Plug with wad of cotton wool (fat-free). 3. Weigh accurately a clean dry 250ml(550ml) Round Bottom Flask. 4. Pour 150ml (300ml) Petroleum Spirit into the flask. 5. Place the thimble containing the sample into a Soxhlet extractor. 6. Connect the Soxhlet extractor to the flask. and a condenser. 7. Place in an Isomantle. 8. Extract for at least 4hr. 9. Distil off Petroleum Spirit. 10. Place the flask in the drying oven for lhr. 11. Remove and place in the desiccator to cool. 12. Reweigh the flask. Calculation Increase in weight of flask x 100 Weight of Sample % = Extractable Fat = 242