The procedure just outlined can be used to isolate and characterize a wide variety of useful mutations. a major limitation of this method is that as stated earlier transposon mutations usually completely disrupt the target gene and therefore lead to a complete inactivation of the gene product. Often we will want to work with point mutations(such as temperature sensitive mutations or nonsense mutations). In the next lecture we will see how transposons can also be used to facilitate analysis and manipulation of point mutationsThe procedure just outlined can be used to isolate and characterize a wide variety of useful mutations. A major limitation of this method is that as stated earlier, transposon mutations usually completely disrupt the target gene and therefore lead to a complete inactivation of the gene product. Often we will want to work with point mutations (such as temperature sensitive mutations or nonsense mutations). In the next lecture we will see how transposons can also be used to facilitate analysis and manipulation of point mutations