第4期 李杰,等植物原生质体培养和体细胞融合技术研究进展 [20]向风宁,夏光敏,周爱芳,等.普通小麦与无芒雀麦不对称体细胞杂交的研究[].植物学报,1999,41 (5):458462 [21] ZHINING Wanga, ROBERT S, ZEMEIRA A, et al Jointed Oatgrass Aegilops cylindrica Hbs )x Wheat Tniticiam aestivum L. ) Hybrids: Hybridization Dynamics in Oregon Wheat Fields[J]. Crop Sci, 2002, 42(6) 18631872 [22 CRIQUI M C. In search of genes involved in the re-entry of mesophyll cells into the cell cycle[ J]. Physiol Pant [23 FOWKEL C. Microtube organization and cell devision in embryogenic protoplast culture of white spruce( picea aa)[J]. Protoplasma,1990,158(1-2):86-94. [24] SIMMONDS D N. Microtubes in cultured protoplast[J]. Acta Bot Neet, 1991, 40(30): 183-1s [25]张承才,彭玲蛋白激酶在植物生长及发育中的作用[A].许智宏,刘春明.植物发育的分子机理 [C]北京:科学出版社,1998.172188 [26 HE GESON J P, HUNT GJ, HABERLACH GT, et al. Somatic hybrids between Solanum brevidens and Solanum tuberosum Expression of a late blight resistance gene and potato leaf roll resistance [J. Plant cell Rep, 1986, 3 212 Advances in the culture of plant protoplast and techniques of somatic hybridization LIJie HUANG Mirren WANG Mirxiu' CAI ru (1. Key Laboratory of Gene Engineer, State Forestry Administration, Nanjing Forestry University, Nanjing 210037, Chi 2. Research Centre of Orchids Engineer, Jiangsu Province, Taizbmu 225321, China Abstract: Since 1970s, the technique of plant protoplast culture and somatic hybridization gradually trend to precision, profoundness, efficiency and diversification. Emphasized particularly on the tech niques of experiment operation and the focuses of recent researches, advances in protoplast culture and somatic hybridization of plants were reviewed in this paper, and their prospects in the applications of theories and practice were also introduced Key words: plant; protoplast culture somatic hybridization; advances 【责任编辑冯元璋】 201994-2008ChinaaCademicJournalElectronicPublishingHouseAllrightsreservedhttp://wwww.cnki.ner[20 ] 向凤宁 ,夏光敏 ,周爱芳 ,等. 普通小麦与无芒雀麦不对称体细胞杂交的研究[J ]. 植物学报 ,1999 ,41 (5) :4582462. [21 ] ZHINING Wanga , ROBERT S , ZEMETRA A , et al Jointed Goatgrass ( Aegilops cylindrica Host) x Wheat ( Triticum aestivum L. ) Hybrids : Hybridization Dynamics in Oregon Wheat Fields[J ]. Crop Sci , 2002 , 42(6) : 186321872. [22 ] CRIQUI M C. In search of genes involved in the re2entry of mesophyll cells into the cell cycle[J ]. Physiol Plant , 1991 ,82(1) :A35. [23 ] FOWKE L C. Microtube organization and cell devision in embryogenic protoplast culture of white spruce( Picea glauca) [J ]. Protoplasma ,1990 ,158(122) :86294. [24 ] SIMMONDS D N. Microtubes in cultured protoplast[J ]. Acta Bot Neet ,1991 ,40(30) :1832195. [25 ] 张承才 ,彭 玲. 蛋白激酶在植物生长及发育中的作用[A ]. 许智宏 ,刘春明. 植物发育的分子机理 [ C]. 北京 :科学出版社 ,1998. 1722188. [26 ] HELGESON J P ,HUNT GJ ,HABERLACH G T ,et al. Somatic hybrids between Solanum brevidens and Solanum tuberosum Expression of a late blight resistance gene and potato leaf roll resistance[J ]. Plant cell Rep ,1986 ,3 : 212. Advances in the culture of plant protoplast and techniques of somatic hybridization LI Jie1 ,2 , HUANG Min2ren 1 , WANG Min2xiu1 , CAI Ru2 (1. Key Laboratory of Gene Engineer , State Forestry Administration , Nanjing Forestry University , Nanjing 210037 ,China ; 2. Research Centre of Orchids Engineer , Jiangsu Province ,Taizhou 225321 , China) Abstract : Since 1970’s ,the technique of plant protoplast culture and somatic hybridization gradually trend to precision ,profoundness ,efficiency and diversification. Emphasized particularly on the tech2 niques of experiment operation and the focuses of recent researches , advances in protoplast culture and somatic hybridization of plants were reviewed in this paper , and their prospects in the applications of theories and practice were also introduced. Key words : plant ; protoplast culture ; somatic hybridization ; advances 【责任编辑 冯元璋】 第 4 期 李 杰 ,等 :植物原生质体培养和体细胞融合技术研究进展 17 © 1994-2008 China Academic Journal Electronic Publishing House. All rights reserved. http://www.cnki.net