Chapter 8 Enzymes:Basic Concepts and Kinetics 2 7 The keat is often referred to as the Ans:f Section:8.4 8 Using attractive forces to lure a substrate into an enzyme site is called the effect. Ans:b Section:8.4 9 Enzymes that do not obey Michaelis-Menten kinetics. Ans:I Section:8.4 10 Notation system used to aid in studying bisubstrate enzyme reactions. Ans:d Section:8.4 Fill in the Blank Questions 1 Enzymes accelerate the rate of a chemical reaction by the free energy of activation of the reaction. Ans:lowering Section:8.3 and Introduction 2 The difference between the standard-state free energy,AG,and the biochemical standard-state free energy is that AGo'refers to the standard free-energy change at Ans:pH 7 Section:8.2 3 Most known enzymes are proteins;however,some molecules have been shown to possess catalytic activity. Ans:RNA Section:Introduction 4 Organic cofactors are referred to as Ans:coenzymes Section:8.1 5 A reaction can occur spontaneously only if AG is Ans:negative Section:8.1 6 When AG for a system is zero,the system is at Ans:equilibrium Section:8.1 7 If an enzyme is inactivated by reaction with iodoacetamide,one can conclude that the enzyme contains a critical residue that is required for activity. Ans:cysteine Section:8.5Chapter 8 Enzymes: Basic Concepts and Kinetics 2 7 The kcat is often referred to as the ____________. Ans: f Section: 8.4 8 Using attractive forces to lure a substrate into an enzyme site is called the ____________ effect. Ans: b Section: 8.4 9 ____________ Enzymes that do not obey Michaelis-Menten kinetics. Ans: l Section: 8.4 10 ____________ Notation system used to aid in studying bisubstrate enzyme reactions. Ans: d Section: 8.4 Fill in the Blank Questions 1 Enzymes accelerate the rate of a chemical reaction by __________________ the free energy of activation of the reaction. Ans: lowering Section: 8.3 and Introduction 2 The difference between the standard-state free energy, ΔGº, and the biochemical standard-state free energy is that ΔGº′ refers to the standard free-energy change at ________. Ans: pH 7 Section: 8.2 3 Most known enzymes are proteins; however, some ___________ molecules have been shown to possess catalytic activity. Ans: RNA Section: Introduction 4 Organic cofactors are referred to as __________________. Ans: coenzymes Section: 8.1 5 A reaction can occur spontaneously only if ΔG is __________________. Ans: negative Section: 8.1 6 When ΔG for a system is zero, the system is at ______________________. Ans: equilibrium Section: 8.1 7 If an enzyme is inactivated by reaction with iodoacetamide, one can conclude that the enzyme contains a critical _________________ residue that is required for activity. Ans: cysteine Section: 8.5