第三军医大学基础部 Data generation 1) mRNA or totaI RNA 1 Raw reads [2 Remove contaminant DNA RNA-Seq 2 Remove artefacts Remove rRNA? 3)Fragment RNA 3 Correct errors(o 4 Re 4 Assemble into transcripts Strand-specific RNA-seq? 岛ms s Post-process transcripts PCR amplification? 6 Select a range of sizes 6) ALign reads to transcripts (7 Sequence cDNA endsRNA-Seq