Transport Processes in Electrophoresis: Electromigration ions migrating in electric field cations->cathode (-ve) anions→anode(+ve) Electroosmosis movement of entire fluid near wall of capillary in one direction! anode (+ve)->cathode (-ve) (A)Analyte dissolved in background electrolyte and pH buffer (B)Silica capillary wall coated with silanol (Si-OH)and Si-O- (C)Wall attracts cations-double-layer forms (D)Cations move towards cathode and sweep fluid in one direction Electroosmotic flow proportional to V-usually greater than electrophoretic flow CEM 333 page 17.6Transport Processes in Electrophoresis: Electromigration ions migrating in electric field cations ® cathode (-ve) anions ® anode (+ve) Electroosmosis movement of entire fluid near wall of capillary in one direction! anode (+ve) ® cathode (-ve) (A) Analyte dissolved in background electrolyte and pH buffer (B) Silica capillary wall coated with silanol (Si-OH) and Si-O- (C) Wall attracts cations - double-layer forms (D) Cations move towards cathode and sweep fluid in one direction Electroosmotic flow proportional to V - usually greater than electrophoretic flow CEM 333 page 17.6