Specification Please note DAKO Sheet Some information s Lot dependent Monoclonal mouse Anti-Human CD62P, P-Selectin, Clone 1E3 Code No, M 7199 Lot 038. Edition 06.12. 00 Antigen synonyms Platelet activation-dependent granule membrane protein(PADGEM)or GMP-140 Introduction The CD62P antigen a 140 kDa transmembrane glycoprotein present on megakaryocytes, activated platelets and activated endothelial cells. In unstimulated platelets and endothelial cells CD62P is stored in the membranes of secretory granules and shows no surface immunostaining with anti-CD62P. When platelets and endothelial cells are activated by agonists such as thrombin, the granules rapidly fuse with the plasma membrane, which will express CD62P on the surface(1-3). CD62P is a receptor for neutrophils and monocytes when it is expressed on activated platelets and endothelium (4) CD62P is a member of the small selectin family of cellular adhesion molecules which also includes CD62E(E-selectin) and CD62L (L-selectin)(5, 6). Its structure, similar to the other members of the selectin family, consists of an N-terminal lectin-like domain of c type(6)followed by an epidermal growth factor-like domain, nine short consensus repeats (SCR)related to those in complement-binding proteins, a transmembrane region and a short cytoplasmic tail which bears no homology to the CD62E and CD62L cytoplasmic domains (3). The major CD62P ligand on neutrophils is the P-Selectin Glycoprotein igand 1, CD162(PSGL-1)(5, 7) Presentation Monoclonal mouse antibody supplied in liquid form as tissue culture supernatant(RPMI 1640 medium containing fetal calf serum)dialysed against 0.05 mol/L Tris/HCL, pH 7.2 containing 15 mmol/L NaN Mouse lg concentration: 170 mg/L Isotype: IgG2a, kappa tal protein concentration 11.7 Storage Clone 1E3.(8 immunogen Fixed thrombin-activated human platelets Specificity/reactivity Anti-CD62P, 1E3, was included in the Sixth International Workshop and Conference on Human Leucocyte Differentiation Antigens( Kobe 1996), and studies by a number of The antibody stains megakaryocytes, activated platelets and activated endothelium. Other normal cells do not react with the antibody Anti-CD62P, 1E3, is useful for assessing platelet activation in acute coronary syndromes by flow cytometry (10)Specification Sheet Please note Some information is Lot dependent. Monoclonal Mouse Anti-Human CD62P, P-Selectin, Clone 1E3 Code No. M 7199 Lot 038. Edition 06.12.00 Antigen synonyms Platelet activation-dependent granule membrane protein (PADGEM) or GMP-140. Introduction The CD62P antigen is a 140 kDa transmembrane glycoprotein present on megakaryocytes, activated platelets and activated endothelial cells. In unstimulated platelets and endothelial cells CD62P is stored in the membranes of secretory granules and shows no surface immunostaining with anti-CD62P. When platelets and endothelial cells are activated by agonists such as thrombin, the granules rapidly fuse with the plasma membrane, which will express CD62P on the surface (1-3). CD62P is a receptor for neutrophils and monocytes when it is expressed on activated platelets and endothelium (4). CD62P is a member of the small selectin family of cellular adhesion molecules which also includes CD62E (E-selectin) and CD62L (L-selectin) (5,6). Its structure, similar to the other members of the selectin family, consists of an N-terminal lectin-like domain of C￾type (6) followed by an epidermal growth factor-like domain, nine short consensus repeats (SCR) related to those in complement-binding proteins, a transmembrane region and a short cytoplasmic tail which bears no homology to the CD62E and CD62L cytoplasmic domains (3). The major CD62P ligand on neutrophils is the P-Selectin Glycoprotein Ligand 1, CD162 (PSGL-1) (5,7). Presentation Monoclonal mouse antibody supplied in liquid form as tissue culture supernatant (RPMI 1640 medium containing fetal calf serum) dialysed against 0.05 mol/L Tris/HCl, pH 7.2 containing 15 mmol/L NaN3. Mouse Ig concentration: 170 mg/L. Isotype: IgG2a, kappa. Total protein concentration: 11.7 g/L. Storage 2-8 °C. Clone 1E3. (8). Immunogen Fixed thrombin-activated human platelets. Specificity/reactivity Anti-CD62P, 1E3, was included in the Sixth International Workshop and Conference on Human Leucocyte Differentiation Antigens (Kobe 1996), and studies by a number of laboratories confirmed its reactivity with the CD62P antigen (9). The antibody stains megakaryocytes, activated platelets and activated endothelium. Other normal cells do not react with the antibody. Anti-CD62P, 1E3, is useful for assessing platelet activation in acute coronary syndromes by flow cytometry (10). (2)