The basis of Agrobacterium mediated genetic engineering T-DNA of A. tumefaciens is excised and integrates into the plant genome as part of the natural infection process Any foreign DNA inserted into the T-DNA Will also be integrated free plasmid A F GH」κLMN Ti-plasmid bacterial T-DNA chromosome D EF G G FE D N integrated pas vir genesThe basis of Agrobacterium￾mediated genetic engineering • T-DNA of A. tumefaciens is excised and integrates into the plant genome as part of the natural infection process. • Any foreign DNA inserted into the T-DNA will also be integrated