Growing DNA Insert Base strand o-P-o-P-o-CH2 (A, T, C or G) Deoxynucleotide triphosphate Sequencing primer OH H e M13 HO O-CH (A, T, C or G) (single strand) Dideoxynucleotide diphosphate OH missing HH Added dideoyynucleotide ddAP ddtp ddcTP ddGTP K|en。 w fragment dNTP Primer CATGGTGCCTCGTAAGCTGA CATGGTGCCTCGAAGCGA GGctassta AAGCTGA CATGG