8/30/2016 Other Factors to Consider Other Factors to Consider Speed of sample processing All aspects of the assay should be easy small sample number-single diagnostic test -technically easy to perform equipment easy to operate results easy to interpret ·Accuracy ·Cost Suitability for the food matrix tested ·fit with company needs -Food constituents should not interfere with training of personnel assay performance ·special equipment Natural microflora should not interfere with service-maintenance contracts accuracy ·disposable supplies Debris should not interfere with accuracy reagents Accepted by industry or government agencies Organizations that Validate Testing Methods for foods AOAC-First or Final Action Status International Standards Organization AOAC International is the most widely rcognized International Dairy Federation and used service that provides testing for manufacturers of test kits AOAC International(formally Association of ·Two programs used Official Analytical Chemists) 、 Collaborative study -publishes FDA Bacteriological Analytical Manual >Rigorous testing >AOAC approved-first action status USDA and FDA do not validate but have manuals that outline the standard methods used in each -Peer-verified organization after 3 years of use-scientific community Food Safety Inspection Service(FSIS)publishes votes for final-action status the Microbiology Laboratory Guidebook Status by AOAC is listed on the kit package 8/30/2016 4 Other Factors to Consider • Speed of sample processing - small sample number - single diagnostic test - large sample large sample number - high-throughput system is needed though more costly • Accuracy • Cost - fit with company needs - training of personnel - special equipment - service – maintenance contracts - disposable supplies - reagents Other Factors to Consider • All aspects of the assay should be easy - technically easy to perform - equipment easy to operate - results easy to interpret • Suitability for the food matrix tested - Food constituents should not interfere with assa y performance - Natural microflora should not interfere with accuracy - Debris should not interfere with accuracy • Accepted by industry or government agencies Organizations that Validate Testing Methods for foods • International Standards Organization • International Dairy Federation • AOAC International (formally Association of Official Analytical Chemists) - publishes FDA Bacteriological Analytical Manual • USDA and FDA do not validate but have manuals that outline the standard methods used in each organization • Food Safety Inspection Service (FSIS) publishes the Microbiology Laboratory Guidebook AOAC - First or Final Action Status • AOAC International is the most widely rcognized and used service that provides testing for manufacturers of test kits • Two programs used - Collaborative study  Rigorous testing  AOAC approved – first action status - Peer-verified  after 3 years of use- scientific community votes for final-action status • Status by AOAC is listed on the kit package