When K2>> K3 M (31) The dissociation constant of the es complex is given by [EJ[S Ro KES (32) k In other words, KM is equal to the dissociation conslant of the ES complex ifk is much smaller than k. When this condition is met, KM is a measure of the strength of the ES complex: a high KM indicates, weak binding; a low KM indicates strong binding. It must be stressed that KM indicates the affinity of the es complex only when ko is much greater than ksWhen K2 >> K3