Poly Acrylamide gels TABLE 6.4 Effective Range of separation of DNAs in Polyacry lamide Gels Effective Acrylamide range of separation Xylene Bromophenol (%[w/v])2 (bp) cyanolFF blue 3.5 1000-2000 460 100 5.0 80-500 260 65 8.0 60-400 160 45 12.0 40-200 70 20 15.0 25-150 60 15 20.0 6-100 45 12 aN, N'-methylenebisacrylamide is included at 1/30th the concentration of acrylamide bThe numbers given are the approximate sizes (in nucleotide pairs)of fragments of double- stranded DNA with which the dye comigratesPolyAcrylamide Gels 8