The overall DNA synthesis reaction: (dNMP)n dNTP(dNMP)n+1 PPi DNA strand substrate elongated DNA strand Replication of both daughter strands proceeds by addition of nucleotide monomers in the 5'to 3'direction Template DNA 5 PP. DNA polymerase B1'B2'B3'B4'B5 B1'B2'B3'B4' B5 61B2B3 0H+ OH ...P attack OH 5→3 Replicated DNA Phosphodiester bond formation The overall DNA synthesis reaction: (dNMP)n + dNTP (dNMP)n+1 + PPi DNA strand substrate elongated DNA strand Phosphodiester bond formation attack Replication of both daughter strands proceeds by addition of nucleotide monomers in the 5’ to 3’ direction