上游充通大学 1.The Exploration of Genes Relies on Key Tools SHANGHAI JIAO TONG UNIVERSITY 限制性内切酶产生的末端 5粘性末端 3粘性末端 平末端 酶 识别序列 酶 识别序列 酶 识别序列 TaqI T/CGA PstI CTGCA/G Alu I AG/CT ClaI AT/CGAT SacI GAGCT/C FnuDII cc/cG MboI /GATC SphI GCATG/C DpnI GA/TC Bgl A/GATCT BdeI GGCGC/C HaeI GG/CC BamHI G/GATCC ApaI GGGCC/C Pvu I CAG/CTG Bc1 T/GATCA KpnI GGTAC/C SmaI Ccc/GGc HindII A/AGCTT NaeI GCC/GGC NooI C/CATGG HpaI GTT/AAC XmaI C/CCGGG NruI TCG/CGA XhoI C/TCGAG BalI TGG/CCA EcoRI G/AATTC MstI TGC/GCA SalI G/TCGAC MhaI TTT/AAA XbaI T/CTAGA EcoRV GAT/ATC限制性内切酶产生的末端 1. The Exploration of Genes Relies on Key Tools