ADRIAMYCIN REMOTE LOADING KIT VIAL 1 VIAL 2 VIAL 3 00PH40 VET 1.0 N NGOH SOUD +o2c03 ADRIAMYCIN 1)ADD CONTENTS OF VIAL 1 TO VLAL 2 AND MIX TO CREATE PH GRADIENT 2)HEAT VIAL 2 AND VIAL 3 AT 00C FOR 5 MINUTES 3)ADD CONTENTS OF VIAL 2 TO VIAL 3 AND HEAT AT 80 C FOR 5 MINUTES TEST VIAL 1.0 ml SALINE 0.1 ml 1.0 N NaOH 4)ADD ONE DROP OF VIAL 3 INTO TEST VIAL AND OBSERVE COLOR RED COLOR:ACCEPT:BLUE COLOR:REJECT Citrate Loading Procedure 300mM Citrate G CeHsO,3- CeHgO,3 3H+ 3H* pH3.5 DOX-NHDOX-NH2 DOX-NHs pH7.514 Citrate Loading Procedure DOX-NH2 pH 7.5 300mM Citrate pH 3.5 C6H5O7 3- 3H+ DOX-NH 2 C6H5O7 3- 3H+ DOX-NH3