Teaching plan of Soochow University Subjects:Biochemistry Experiment Class:2019 Pharmaceutical sciences Date:2020.11. 06/Fri/5-8 Chapter Text page:N/A Teaching duration:200 Title The Lowry Assay for Protein quantification 1.Understand the principle of centrifugation and know how to manipulate centrifuge Teaching goal 2.Understand Beer-Lambert Law and the method to measure substance concentration using spectrophotometer 3.Learn to use pipets l、Content: (1),Principle of Lowry assay 20 (2),Procedure of protein measurement 30 Teaching content、 (3).Review how to use of spectrophotometer 10 Teaching nethod、 (4).Student practices 140 Teaching emphasis、 difficulty、 2、 Key point: (1).Principle of the Lowry assay time (2).Understand the linear range of the assay distribution (3).Copper reagent need to be prepared fresh.Upon adding reagent II need to be mixed immediately Teaching method:Teacher should explain and display the experiment according to powerpointTeaching plan of Soochow University Subjects：Biochemistry Experiment Class：2019 Pharmaceutical sciences Date：2020. 11. 06/Fri/5-8 Chapter 1 Text page：N/A Teaching duration: 200 Title The Lowry Assay for Protein quantification Teaching goal 1.Understand the principle of centrifugation and know how to manipulate centrifuge 2.Understand Beer-Lambert Law and the method to measure substance concentration using spectrophotometer 3. Learn to use pipets Teaching content、 Teaching method、 Teaching emphasis、 difficulty、 time distribution 1、 Content： （1）、Principle of Lowry assay 20‘ （2）、Procedure of protein measurement 30‘ （3）、Review how to use of spectrophotometer 10’ （4）、Student practices 140’ 2、 Key point： (1). Principle of the Lowry assay (2). Understand the linear range of the assay (3). Copper reagent need to be prepared fresh. Upon adding reagent II need to be mixed immediately Teaching method: Teacher should explain and display the experiment according to powerpoint