8/30/2016 US Food Micro Market-Test Volume Testing for pathogens and spoilage microbes is essential to ensure safe and wholesome food Conventional test methods -sensitive cost effective labor intensive 00a require days to complete Products that are minimally processed have a short half-life conventional methods are not reasonable to use due to the short shelf life Rapid methods may be more appropriate Flowchart showing When considering whether an assay is processing steps and "rapid",all steps in the protocol must be relative time needed to considered detect a pathogen in a The rapid assay may only take 15 min food sample. A preenrichment and/or selective enrichment may be needed IMS-immune-magnetic separation Enrichment may take days before the 15 Particles with magnetic min rapid assay can be performed properties are modified Rapid tests have detection limits ranging with target-specific from 102 to 105 CFU/g or ml antibody to capture and purify the target using a magnetic field. 8/30/2016 2 • Testing for pathogens and spoilage microbes is essential to ensure safe and wholesome food • Conventional test methods - sens t ve i i - cost effective - labor intensive - require days to complete • Products that are minimally processed have a short half-life - conventional methods are not reasonable to use due to the short shelf life • Rapid methods may be more appropriate • When considering whether an assay is ”rapid”, all steps in the protocol must be considered • The rapid assay may only take 15 min • A preenrichment and/or selective enrichment may be needed • Enrichment may take days before the 15 min rap y id assay can be performed • Rapid tests have detection limits ranging from 102 to 105 CFU/g or ml Flowchart showing processing steps and relative time needed to detect a pathogen in a food sample. IMS - immune-magnetic separation Particles with magnetic properties are modified with target-specific antibody to capture and purify the target using a magnetic field