Nutrient requirements of bacterial growth water Carbon source Nitrogen source Minerals Growth factors organic compounds such as amino acids purines 嘌呤, pyrimidines嘧啶, and vitamins that a cell must have for growth but cannot synthesize itself.Nutrient requirements of bacterial growth • water • Carbon source • Nitrogen source • Minerals • Growth factors: organic compounds such as amino acids, purines 嘌呤, pyrimidines嘧啶, and vitamins that a cell must have for growth but cannot synthesize itself