BEH.462/3.962J Molecular Principles of Biomaterials Spring 2003 Applications of tiny drug carriers and cellular markers Applications Delivery to tissues from circulation tic drugs Detect tumors Intracellular delivery Va class I MHC loading-priming CD8 o Gene delivery Delivery of plasmid DNA Shutting off production of certain proteins by delivery of anti-sense oligonucleotides to bind bosomal mRNA o Intracellular toxins for cancer therapy o Ribozyme delivery drug delivery to organe drug delivery to mitochondria Objectives protection of cargos from degradation o e.g. DNA protection from DNAses o protein protection from proteases, phosphatases avoid opsonization and production of antibodies against drug molecule o opsonization: coating foreign protein, small molecule, or particle with antibodies or complement proteins leads to macrophage binding and destruction sourceofopsonizationanimationhttp://medtech.cls.msu.edu/isl/immunology/OpSoniZe.htm Lecture 14-- and micro-particles 2 of 6BEH.462/3.962J Molecular Principles of Biomaterials Spring 2003 Applications of tiny drug carriers and cellular markers Applications ƒ Delivery to tissues from circulation o Therapeutic drugs ƒ Anti-cancer drugs 1 o Markers for analysis/detection ƒ Detect tumors ƒ Infected cells ƒ Anti-pathogen lymphocytes ƒ Intracellular delivery o Vaccines ƒ class I MHC loading – priming CD8+ T cells o Gene delivery ƒ Delivery of plasmid DNA o Anti-sense therapy ƒ Shutting off production of certain proteins by delivery of anti-sense oligonucleotides to bind ribosomal mRNA o Intracellular toxins for cancer therapy o Ribozyme delivery ƒ ? o drug delivery to organelles 2 ƒ drug delivery to mitochondria Objectives ƒ protection of cargos from degradation o e.g. DNA protection from DNAses o protein protection from proteases, phosphatases ƒ avoid opsonization and production of antibodies against drug molecule o opsonization: coating foreign protein, small molecule, or particle with antibodies or complement proteins ƒ leads to macrophage binding and destruction ƒ source of opsonization animation: http://medtech.cls.msu.edu/ISL/immunology/opsonize.htm Lecture 14 – nano- and micro-particles 2 of 6