Millstein Image removed due to copyright reasons FROM NOBEL LECTURE 1984 in vitro synthesis of immunoglobulin light chains To our delight we ran into the unexpected observation of the existence of a biosynthetic precursor of light chains. Further experiments led us to propose the extra N-terminal sequence was a signal for vectorial transport across the membrane during protein synthesis. That was the first evidence which indicated that the signal for secretion was an n-terminal segment, rapidly cleaved during protein synthesisMillstein “in vitro synthesis of immunoglobulin light chains. … To our delight we ran into the unexpected observation of the existence of a biosynthetic precursor of light chains. Further experiments led us to propose the extra N-terminal sequence was a signal for vectorial transport across the membrane during protein synthesis. That was the first evidence which indicated that the signal for secretion was an N-terminal segment, rapidly cleaved during protein synthesis.” FROM NOBEL LECTURE 1984 Image removed due to copyright reasons