Differential Labelling Schemes and Isotope Filtering Nomenclature Labollod with N Unlabelled C,N 38eot8peeee tope-separated ←→ isotope-filtered 2D isotope-filtered(f1), 3D ope-edit Cdc42 ACK Free 15N-abelled ACK 15N-ACK+unlabelled Cdc42 1120 140 14. 16.0 118.0 120.0 1220 12 1240 124.0 126.0 126. 05b0508507店70ds 0.50050085&.051065Differential Labelling Schemes and Isotope Filtering H H 13C H 13C 12C H 12C Labelled with 13C, 15N Unlabelled 12C, 14N 15N H 2D isotope-edited 3D isotope-separated isotope-filtered 2D isotope-filtered (f1), isotope-edited (f2) 3D isotope-filtered (f1), isotope-separated (f2/f3) H 14N Nomenclature Cdc42 + ACK Cdc42/ACK Complex Free 15N-labelled ACK 10.5 10.0 9.5 9.0 8.5 8.0 7.5 7.0 6.5 10.5 10.0 9.5 9.0 8.5 8.0 7.5 7.0 6.5 15N-ACK + unlabelled Cdc42 110.0 112.0 114.0 116.0 118.0 120.0 122.0 124.0 126.0 110.0 112.0 114.0 116.0 118.0 120.0 122.0 124.0 126.0