Principle of collection asepsIs avoid contamination by normal flora or bacteria from environment 2. take the correct specimen specimens include blood, urine, pus, stool, sputum, CSF, or other material, as indicated by the localization of the disease process 3. early before using antibiotics 4. fresh test ASAP (As Soon as possiblePrinciple of collection 1． 2. 3． ． asepsis avoid contamination by normal flora or bacteria from environment. take the correct specimen specimens include blood, urine, pus, stool, sputum, CSF, or other material, as indicated by the localization of the disease process. early before using antibiotics 4 fresh test ASAP（As Soon As Possible ）