TLC all of the fractions from your column, have your tlC plates checked by an instructor, and reproduce them in your lab notebook Calculate r values for the three compounds in the chosen TLC solvent mixture Results To obtain your EE Rating"in Purification by Flash Column Chromatography, you must successfully separate all three components of the mixture by tlC and correctly calculate the corresponding R values• TLC all of the fractions from your column, have your TLC plates checked by an instructor, and reproduce them in your lab notebook. • Calculate R f values for the three compounds in the chosen TLC solvent mixture. Results: • To obtain your "EE Rating" in Purification by Flash Column Chromatography, you must successfully separate all three components of the mixture by TLC and correctly calculate the corresponding Rf values. 31