One face of the promoter contains the contact points for RNA -35 sequence -10 sequence ↓44↓↓ ↓↓↓↓↓ mOdification prevents contemplate stran RNA polymerase binding +t **t uNwinding f RNA polymerase XXXXXX AACTG T XXXXXXXXXXXXXXXXXAT Template strand XXXXX TTAXXXXXXX AX Mutations abolish or educe promoter actvty Most points of contact lie on one face of DNA (on the nontemplate strand)One face of the promoter contains the contact points for RNA