ARTICLE IN PRESS O. Roualdes et al. Biomaterials xxx(2009)1-12 a10 3.5 3.0 oE 左02 0.5 0.0 Days c6.5 d45 5.5 盟5.0 4.5 3.0 4.0 332 2.0 510 1.0 函 Days f7.5 10 55 7 5544 86 635 3.0 2.5 2.0 505 自爵自 Fig 3. Fibroblasts(a c, e)and osteoblasts(b, d, f) were cultured for 3, 6 and 10 days on rough C or polished (2 ceramic and on plastic(control E under standard cell culture conditions in 24-well culture plates At each time of culture, cell proliferation was assessed using the mit assay (a, b)and culture media were harvested for human type I collagen(c. d)and human fibronectin(e f) synthesis quantification Values are expressed as mean+SEM, n=9: +p<0.05, +tp<0.01, +++p<0.001(compared to control cultures a normal knee articulation associated with a normal articular capsul The histological appearances of the synovial membrane were However, a fine and granular deposit was observed around the patella normal at low magnification (5x). For control and particles for some animals that received particles. It certainly injected groups, no alteration of the synovial membrane sponded of the accumulation of the powder in the tissues thickness or hyperplasia could be observed Please cite this article in press as: Roualdes 0, et al, In vitro and in vivo evaluation of an, Biomaterials(2009). doi: 10.1016/ j biomaterials 2009. 11.107a normal knee articulation associated with a normal articular capsule. However, a fine and granular deposit was observed around the patella for some animals that received ceramic particles. It certainly corre￾sponded of the accumulation of the powder in the tissues. The histological appearances of the synovial membrane were normal at low magnification (5). For control and particles injected groups, no alteration of the synovial membrane thickness or hyperplasia could be observed. 3 6 10 0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0 ++ ++ a Days 3 6 10 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 ++ +++ + + ++ b Days Cell proliferation (optical density) Cell proliferation (optical density) Human type I collagen (µg/mL) Human type I collagen (µg/mL) Human fibronectin (µg/mL) Human fibronectin (µg/mL) 3 6 10 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 ++ c Days 3 6 10 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 Days d 3 6 10 0 1 2 3 4 5 6 7 8 9 10 11 e Days 3 6 10 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 + f Days Fig. 3. Fibroblasts (a, c, e) and osteoblasts (b, d, f) were cultured for 3, 6 and 10 days on rough ( ) or polished ( ) ceramic and on plastic (control, ) under standard cell culture conditions in 24-well culture plates. At each time of culture, cell proliferation was assessed using the MTT assay (a, b) and culture media were harvested for human type I collagen (c, d) and human fibronectin (e, f) synthesis quantification. Values are expressed as mean  SEM, n ¼ 9; þp < 0.05, þþp < 0.01, þþþp < 0.001 (compared to control cultures). O. Roualdes et al. / Biomaterials xxx (2009) 1–12 5 ARTICLE IN PRESS Please cite this article in press as: Roualdes O, et al., In vitro and in vivo evaluation of an..., Biomaterials (2009), doi:10.1016/ j.biomaterials.2009.11.107