同尾酶生产的粘性末端的连接 5" TGATCA GGATCC GGATCC ACTAGT 5 CCTAGG CCTAGG Bcll 5" 5 GATCA 5 GATCC G ACTAG 5 5 G CCTAG 5 退火 5" T GATCC G GATCA ACTAG G CCTAG T 5 T GATCC G GATCA ACTAG G CCTAG T 5 T4-DNA ligase TGATCC GGATCA ACTAGG CCTAGT TGATCC GGATCA ACTAGG CCTAGT同尾酶生产的粘性末端的连接 BamHI 5' 5' GGATCC CCTAGG GGATCC CCTAGG 5' G CCTAG GATCC G 5' T 5' ACTAG 5' 5' GATCA T 5' 退火 G CCTAG GATCC G 5' T ACTAG 5' GATCA T T4-DNA ligase TGATCC ACTAGG 5' 5' GGATCA CCTAGT 5' TGATCA ACTAGT 5' BclI G CCTAG GATCC G 5' T ACTAG 5' GATCA T TGATCC ACTAGG 5' 5' GGATCA CCTAGT