第七章序列比对和数据库搜索 页码,6/28 具( Pearson,1996)。对于比对多模块的蛋白质而言,寻找次优比对尤为重要。正如图7.6所 示, LALI GN程序被用来获得三个最好的局部比对(比对人类凝血因子X和因子Ⅺ1)。一个标 准的 Smi th- waterman算法只会报告出最好的一个比对,改良的算法会报告出第二和第三的比 对方式,从而显示出功能结构域 Compari son of A. fg-human. aa >f9 gi 119772 sp P00740 FA9-_HUMAN COAGULATI ON FA-461 aa B. f12-hum. aa>f12 gi 119763 sp P00748 FA12_HUMAN COAGULATI ON-615 aa usi ng protei n matri x O 35. 4% i denti ty in 254 aa overI ap; score: 358 220230240250260270 F9 OSENDETRVVGGEDAKPGOFPWOVVLNGKVDAFCGGSI VNEKWI VTAAHCVE---TGVKI F12 KSLSSMTRVVGGLVALRGAHPYI AALY-WGHSFCAGSLI APCWVLTAAHCLODRPAPEDL 370380390400410420 280290300310320330 F9 TVVAGEHNI EETEHTEOKRNVI RI I PHHNYNAAI NKYNHDI ALLELDEPL-----VLNSY F12 TWLGOERRNHSCEPCQTLAVRSYRLHEAFSPV--SYOHDLALLRLOEDADGSCALLSPY 430440450460470480 340350360370380 F9 VTPI CLADKEYTNI FLKFGSGYVSGWGRVFHKGRS-ALVLOYLRVPLVDRATCLRSTKF F12 VOPVCLPSGAARPSETTLCO-VAGWGHOFEGAEEYASFLQEAQVPFLSLERCSAPDVHG 490500510520530 390400410420430440 F9-TIYNNMFCAGFHEGGRDSCQGDSGGPHVTEVEGTS---FLTGI I SWGEECAMKGKYGIY F12 SSI LPGMLCAGFLEGGTDACOGDSGGPLVCEDOAAERRLTLQGI I SWGSGCGDRNKPGVY file://E:wcb生物信息学(中译本)\第七章序列比对和数据库搜索.htm 2005-1-18Ꮉ݋)Pearson,1996)DŽᇍѢ↨ᇍ໮῵ഫⱘ㲟ⱑ䋼㗠㿔ˈᇏᡒ⃵Ӭ↨ᇍᇸЎ䞡㽕DŽℷབ೒7.6᠔ ⼎ˈLALIGN⿟ᑣ㹿⫼ᴹ㦋ᕫϝϾ᳔དⱘሔ䚼↨ᇍ˄↨ᇍҎ㉏ޱ㸔಴ᄤIX੠಴ᄤXII˅DŽϔϾᷛ ޚⱘSmith-watermanㅫ⊩াӮ᡹ਞߎ᳔དⱘϔϾ↨ᇍˈᬍ㡃ⱘㅫ⊩Ӯ᡹ਞߎ㄀Ѡ੠㄀ϝⱘ↨ ᇍᮍᓣˈҢ㗠ᰒ⼎ࡳߎ㛑㒧ᵘඳDŽ Comparison of: A. f9-human.aa >f9 gi|119772|sp|P00740|FA9_HUMAN COAGULATION FA -461 aa B. f12-hum.aa>f12 gi|119763|sp|P00748|FA12_HUMAN COAGULATION -615 aa using protein matrix ķ 35.4% identity in 254 aa overlap; score: 358 220 230 240 250 260 270 F9 QSFNDFTRVVGGEDAKPGQFPWQVVLNGKVDAFCGGSIVNEKWIVTAAHCVE---TGVKI .:....:::::: : .:. :. ..: ..::.::... :..:::::.. . .. F12 KSLSSMTRVVGGLVALRGAHPYIAALY-WGHSFCAGSLIAPCWVLTAAHCLQDRPAPEDL 370 380 390 400 410 420 280 290 300 310 320 330 F9 TVVAGEHNIEETEHTEQKRNVIRIIPHHNYNAAINKYNHDIALLELDEPL-----VLNSY ::: :... ... .. :. .: . :...... .:.::.::: :.: .:..: F12 TVVLGQERRNHSCEPCQTLAVRSYRLHEAFSPV--SYQHDLALLRLQEDADGSCALLSPY 430 440 450 460 470 480 340 350 360 370 380 F9 VTPICIADKEYTNIFLKFGSGYVSGWGRVFHKGRS-ALVLQYLRVPLVDRATCLRSTKF- : :.:... . .. :.:::. :. . . : :: .::... . : .. F12 VQPVCLPSGAARPSETTLCQ—VAGWGHQFEGAEEYASFLQEAQVPFLSLERCSAPDVHG 490 500 510 520 530 390 400 410 420 430 440 F9 -TIYNNMFCAGFHEGGRDSCQGDSGGPHVTEVEGTS---FLTGIISWGEECAMKGKYGIY .: .:.:::: ::: :.:::::::: : : .... : ::::::..:. ..: :.: F12 SSILPGMLCAGFLEGGTDACQGDSGGPLVCEDQAAERRLTLQGIISWGSGCGDRNKPGVY ㄀ϗゴᑣ߫↨ᇍ੠᭄᥂ᑧ᧰㋶ 义ⷕˈ6/28 file://E:\wcb\⫳⠽ֵᙃᄺ˄Ё䆥ᴀ˅?㄀ϗゴᑣ߫↨ᇍ੠᭄᥂ᑧ᧰㋶.htm 2005-1-18 Click to buy NOW! PDF-XCHANGE www.docu-track.com Click to buy NOW! PDF-XCHANGE www.docu-track.com