Nuclear Magnetic Resonance Spectroscopy When nuclei that are aligned parallel with an external magnetic field are irradiated with the proper frequency of electromagnetic radiation the energy is absorbed and the nuclei“spin-flips”to the higher-energy antiparallel alignment Nuclei that undergo "spin-flips"in response to applied radiation are said to be in resonance with the applied radiation -nuclear magnetic resonance Frequency necessary for resonance depends on strength of external field and the identity of the nucleiWhen nuclei that are aligned parallel with an external magnetic field are irradiated with the proper frequency of electromagnetic radiation the energy is absorbed and the nuclei “spin-flips” to the higher-energy antiparallel alignment ▪ Nuclei that undergo “spin-flips” in response to applied radiation are said to be in resonance with the applied radiation - nuclear magnetic resonance ▪ Frequency necessary for resonance depends on strength of external field and the identity of the nuclei Nuclear Magnetic Resonance Spectroscopy