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pled to neocarzinostatin (SMANCS)into the hepatic artery peutics,it is vital that novel technologies be developed to for to treat liver cancer is another example of a polymer drug an be achieved b et al 2001;Tor e202w PP otein hepa id. wh ute.8 for the cutaneous injection (Okada et 1982)Emphasis has been bicin for treating in B for fu aposi's s coma and lip placed on increasing the pends on or protein s appeared in the pharmace perties of th ry formulations.The se formulations ve due to its ise of tion.Howe ver.there ar ugh the li r the on in th rst pa the st ach and be de been much attention on chrono tiss stems are inte incre with the ogical rh syste d in dal ield of r given new ways to treat a nun 198 th the (Le e,1991).More must re Clearly,the distinc ties of each individual ition.them sal lining that u ally prot cts organs fron abra eae Thus, and protein should b ften involves amsof polymer che ount for the drug's unigue pharma codvnamics and pharma aceutical scientists (Robinson 1997) oral ro a subject o nuous intensive arch efforts i hat can re d to a ph chang eand,at the san among th are th rug sucn as na outes with nistration being con line pH of the (Figure 3).One of th no eltic tein from degradation in the small in ine and tempor instability and rmeability of these dru En Morishita et al 2002.Fig e 4 sh s the nain ellular bar activity in the int rs to drug or prote mech cant advances in the creation of protein and peptide thera AIChE Journal December 2003 Vol.49.No.12 2995pled to neocarzinostatin SMANCS into the hepatic artery Ž . to treat liver cancer is another example of a polymer drug targeting strategy Maeda, 1991 . Ž . Specific targeting to specific tissues can be achieved by coupling the polymer-drug with a molecule such as antibody Ž or a carbohydrate recognized by tissue cell surface recep- . tors. One challenge has been discovering appropriate target￾ing molecules. Galactose which is recognized by the hepato￾cyte cell surface asialoglycoprotein receptor is one example where successful targeting has been achieved Duncan, 2003 . Ž . Liposomes have been used both for targeting and to create safer intravenous drug formulations for drugs such as doxoru￾bicin for treating HIV-associated Kaposi’s sarcoma and lipo￾somal amphotericin B for fungal infections in cancer Park et Ž al., 1997; Torchilin, 1994 . Liposomes provide a way of alter- . ing a drugs pharmacokinetics and can make them less toxic by changing their biodistribution in the body. Nonin©asi©e deli©ery of proteins A significant opportunity has appeared in the pharmaceu￾tical sciences over the past 35 years with the development of advanced drug delivery formulations. These formulations do not simply release the drug, peptide, or protein at some char￾acteristic rate, but do so in a way that the pharmaceutical scientist and molecular designer wants Peppas et al., 2000 . Ž . For example, insulin may be delivered only when needed, calcitonin may be directed to bypass the stomach and be de￾livered only in the upper small intestine, and large molecular weight, genetically-engineered molecules are delivered across tissues at acceptable rates Peppas and Huang, 2002 . Ž . The increased availability of large molecular weight pro￾tein- and peptide-based drugs with the recent advances in the field of molecular biology has given new ways to treat a num￾ber of diseases Silbart and Keren, 1989 . The structure, Ž . physicochemical properties, stability, pharmacodynamics, and pharmacokinetics of these new biopharmaceuticals place stringent demands on the way they are delivered into the body Ž . Lee, 1991 . More specifically, peptides and proteins must re￾tain their structural integrity until they reach their delivery site and cannot be degraded upon enzymatic interactions. In addition, the mucosal lining that usually protects organs from mechanical and abrasive damage poses a main obstacle for the successful penetration of such drugs to the appropriate delivery site. Thus, each peptide and protein should be ad￾ministered in an optimal temporal pattern that would ac￾count for the drug’s unique pharmacodynamics and pharma￾cokinetics. Alternative delivery methods to the traditional intravenous route are a subject of continuous intensive research efforts in both industry and academia. Prominent among these are the nasal, transdermal, pulmonary, buccal, ocular, vaginal, rectal and oral delivery routes with oral administration being con￾sidered the most convenient Lehr, 1994 . Ž . The oral delivery route is the most challenging for complex drugs such as peptides or proteins from a technical perspec￾tive due to the extremely low bioavailability associated with the instability and low permeability of these drugs. Enzyme activity in the intestine or stomach also contributes signifi￾cantly to the overall instability of such drugs. With the signifi￾cant advances in the creation of protein and peptide thera￾peutics, it is vital that novel technologies be developed to for￾mulate and deliver these drugs. Important challenges relate to the problems of stability, low bioavailability, the degree and rate at which a substance as a drug is absorbed into a Ž . living system or is made available at the site of physiological activity and short half-life of proteins and peptides Peppas Ž et al., 2001; Torres-Lugo et al., 2002b .. For example, the bioavailability of leuprolide, a luteinizing hormone-releasing hormone LHRH analog, is only 0.05% Ž . through the oral route, 3% for the nasal route, 8% for the rectal route, 38% for the vaginal route, and 65% for the sub￾cutaneous injection Okada et al., 1982 . Emphasis has been Ž . placed on increasing the bioavailability of peptides and pro￾teins for various administration routes by designing novel drug delivery systems tailored specifically for a specific application Ž . Lowman et al., 1999; Kim and Peppas, 2002; Peppas, 2003 . Typically, the selection of the route of administration de￾pends on  Intended therapeutic use of a peptide or protein  Desired duration of service-life  Physicochemical properties of the protein or peptide. For example, oral delivery of peptides and proteins is at￾tractive due to its ease of application. However, there are many problems that need to be addressed, such as the harsh gastric environment, permeability through the lipophilic ep￾ithelial cellular membrane andror the tight junction in the upper small intestine, as well as first pass metabolism by the liver. Recently, there also has been much attention on chrono￾pharmacological drug delivery systems. These drug delivery systems are intended to match the delivery of the therapeutic agents with the biological rhythm. These systems are impor￾tant especially in the area of endocrinology and in delivery of vaccines. For example, it has been shown that the treatment of hypopituitary dwarfism by administration of human growth hormone releasing hormone GHRH is more effective when Ž . the GHRH is administered in a pulsatile manner Chappel, Ž 1999 .. Clearly, the distinct properties of each individual peptide and protein combined with the physiological peculiarities of each delivery route prevent the design of a generic con￾trolled-release system for even a general subset of peptides or proteins. Furthermore, the complexity involved in the de￾sign of a controlled-release device is very interdisciplinary and often involves teams of polymer chemists, chemical engi￾neers, pharmacokineticists, pharmacologists, clinicians, and pharmaceutical scientists Robinson, 1997 . Ž . In the oral route, Peppas and co-workers Lowman et al., Ž 1999a,b; Torres-Lugo et al., 2002a,b have used bioadhesives . that can respond to a pH change and, at the same time, pro￾tect protein drugs such as insulin and calcitonin from the acidic pH of the stomach and then release it into the more alkaline pH of the intestine Figure 3 . One of the novelties Ž . of these particular bioadhesive polymers is that they are also able to protect the protein from degradation in the small in￾testine and temporarily open connections between intestinal cells to allow proteins to penetrate into the intestine Ž . Morishita et al., 2002 . Figure 4 shows the main cellular bar￾riers to drug or protein transport. Several mechanisms are involved in protein cellular transport including paracellular, transcellular and transcytotic transport Figure 5 . As Ž . AIChE Journal December 2003 Vol. 49, No. 12 2995
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