Termination of a bacterial transcript Transcription is a discontinuous process, with the polymerase pausing regularly and making a choice between continuing elongation or terminating Which choice is depends on which alternative is more favorable in the thermodynamic terms Bacteria appear to use two distinct strategies for transcription termination In the template strand, about half the terminating position contains an inverted palindrome followed by a run of deoxyadenosine nucleotides. Intrinsic terminators Rho dependentTermination of a bacterial transcript • Transcription is a discontinuous process, with the polymerase pausing regularly and making a “choice” between continuing elongation or terminating. • Which choice is depends on which alternative is more favorable in the thermodynamic terms. • Bacteria appear to use two distinct strategies for transcription termination. • In the template strand, about half the terminating position contains an inverted palindrome followed by a run of deoxyadenosine nucleotides. Intrinsic terminators. • Rho dependent