1. Proteins in native conformation can be purified (separated) according to their size, solubility, charge, and binding aflinity 1.1 Proteins can be separated from small molecules by dialysis thanon a semipermeable membranes 1.1.1 Molecules significantly larger than the diameter of the membrane pores are retained in the dialysis bag, whereas small ones diffuse out1. Proteins in native conformation can be purified (separated) according to their size, solubility, charge, and binding affinity. 1.1 Proteins can be separated from small molecules by dialysis through a semipermeable membranes. 1.1.1 Molecules significantly larger than the diameter of the membrane pores are retained in the dialysis bag, whereas small ones diffuse out