Substituting equation 27 into equation 26 yields the Michaelis-Menten e [S] max [S]+ KM (28) This equation accounts for the kinetic data given in Figure 8-15.At very low substrate concentration, when [S] is much less than KM, V [s]Vmax/KMi that is, the rate is directly proportional to the substrate concentration. At high substrate concentration, when [S] is much greater than KM, V=Vmax; that is, the rate is maximal, independent of substrate concentration. When s<< Km, V=SImar/K When s>> Kmg V=VWhen [S] << Km, V = [S] Vmax/Km When [S] >> Km, V = Vmax