Polymerase Chain Reaction(PCR) DNA to be amplified 5 and 3 primers DNA co py Cy cle numb e ST EP 1: Heat-denature dna agarose or sepande ge/p Verification of PCR product STEP 2: Cool-a neal primers skb STEP 3: Polym erase extend/elongate 1857 EP 1: Heat-denature DNA STEP 2: Cool-a neal primers 383 12345 300 STEP 3: Polymerase Extend/elongate ladder PCR fragmentsPolymerase Chain Reaction (PCR)